NbThermo: a new thermostability database for nanobodies

Mario S. Valdés-Tresanco, Mario E. Valdés-Tresanco, Esteban Molina-Abad, Ernesto Moreno

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


We present NbThermo-a first-in-class database that collects melting temperatures (Tm), amino acid sequences and several other categories of useful data for hundreds of nanobodies (Nbs), compiled from an extensive literature search. This so-far unique database currently contains up-to-date, manually curated data for 564 Nbs. It represents a contribution to efforts aimed at developing new algorithms for reliable Tm prediction to assist Nb engineering for a wide range of applications of these unique biomolecules. Nbs from the two most common source organisms-llama and camel-show similar distributions of melting temperatures. A first exploratory research that takes advantage of this large data collection evidences that understanding the structural bases of Nb thermostability is a complex task, since there are no apparent differences in sequence patterns between the frameworks of Nbs with lower and higher melting temperatures, indicating that the highly variable loops play a relevant role in defining Nb thermostability. Database URL https://valdes-tresanco-ms.github.io/NbThermo.

Original languageEnglish
JournalDatabase : the journal of biological databases and curation
StatePublished - 12 Apr 2023

Product types of Minciencias

  • A1 article - Q1


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