A proteomic approach to the mechanisms underlying activation of aluminium resistance in roots of Urochloa decumbens

Catalina Arroyave, Roser Tolrà, Livia Chaves, Marcelo Claro de Souza, Juan Barceló, Charlotte Poschenrieder

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

5 Citas (Scopus)

Resumen

© 2017 Elsevier Inc. The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72–96 h and is preceded by a sensitive phase (24–48 h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96 h exposure to 0 or 200 μM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase. Only 11 proteins revealed Al-induced abundance differences; six were identified. After 24 h, phenylalanine ammonium lyase (PAL), methionine synthase (MS), and deoxymugineic acid synthase (DMAS) decreased, while acid phosphatase (APase) abundance increased. Coincident with growth recovering, PAL and MS, but not DMAS, returned to initial levels. After 96 h, γ‑carbonic anhydrase (γ‑CA) and adenylate kinase (AK) along with two unidentified proteins were more abundant. In conclusion, few protein changes characterize the initial response to Al in signalgrass. During the alarm phase, changes are related to P-mobilization, downregulation of Fe-acquisition, reduction of phenolic biosynthesis, and small stimulation of organic acid exudation. After recovering (resistant phase), biosynthesis of phenolics and methionine, but not Fe-mobilization are re-established. Full expression of Al-resistance is characterized by enhanced γ‑CA mediating mitochondrial complex I assembly and increased AK abundance indicating higher root respiration and better provision of ADP and Mg2 +to ATP synthase, respectively. The unidentified proteins and the specific role of γ‑CA in Al resistance of U. decumbens will centre future research.
Idioma originalInglés estadounidense
Páginas (desde-hasta)145-151
Número de páginas7
PublicaciónJournal of Inorganic Biochemistry
DOI
EstadoPublicada - 1 abr 2018

Huella dactilar

Aluminum
Proteomics
Carbonic Anhydrases
Chemical activation
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Adenylate Kinase
Lyases
Biosynthesis
Phenylalanine
Ammonium Compounds
Acids
Proteins
Oxalates
Organic acids
Exudates and Transudates
Proteome
Growth
Acid Phosphatase
Citric Acid
Methionine

Citar esto

Arroyave, Catalina ; Tolrà, Roser ; Chaves, Livia ; de Souza, Marcelo Claro ; Barceló, Juan ; Poschenrieder, Charlotte. / A proteomic approach to the mechanisms underlying activation of aluminium resistance in roots of Urochloa decumbens. En: Journal of Inorganic Biochemistry. 2018 ; pp. 145-151.
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abstract = "{\circledC} 2017 Elsevier Inc. The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72–96 h and is preceded by a sensitive phase (24–48 h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96 h exposure to 0 or 200 μM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase. Only 11 proteins revealed Al-induced abundance differences; six were identified. After 24 h, phenylalanine ammonium lyase (PAL), methionine synthase (MS), and deoxymugineic acid synthase (DMAS) decreased, while acid phosphatase (APase) abundance increased. Coincident with growth recovering, PAL and MS, but not DMAS, returned to initial levels. After 96 h, γ‑carbonic anhydrase (γ‑CA) and adenylate kinase (AK) along with two unidentified proteins were more abundant. In conclusion, few protein changes characterize the initial response to Al in signalgrass. During the alarm phase, changes are related to P-mobilization, downregulation of Fe-acquisition, reduction of phenolic biosynthesis, and small stimulation of organic acid exudation. After recovering (resistant phase), biosynthesis of phenolics and methionine, but not Fe-mobilization are re-established. Full expression of Al-resistance is characterized by enhanced γ‑CA mediating mitochondrial complex I assembly and increased AK abundance indicating higher root respiration and better provision of ADP and Mg2 +to ATP synthase, respectively. The unidentified proteins and the specific role of γ‑CA in Al resistance of U. decumbens will centre future research.",
author = "Catalina Arroyave and Roser Tolr{\`a} and Livia Chaves and {de Souza}, {Marcelo Claro} and Juan Barcel{\'o} and Charlotte Poschenrieder",
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language = "American English",
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A proteomic approach to the mechanisms underlying activation of aluminium resistance in roots of Urochloa decumbens. / Arroyave, Catalina; Tolrà, Roser; Chaves, Livia; de Souza, Marcelo Claro; Barceló, Juan; Poschenrieder, Charlotte.

En: Journal of Inorganic Biochemistry, 01.04.2018, p. 145-151.

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

TY - JOUR

T1 - A proteomic approach to the mechanisms underlying activation of aluminium resistance in roots of Urochloa decumbens

AU - Arroyave, Catalina

AU - Tolrà, Roser

AU - Chaves, Livia

AU - de Souza, Marcelo Claro

AU - Barceló, Juan

AU - Poschenrieder, Charlotte

PY - 2018/4/1

Y1 - 2018/4/1

N2 - © 2017 Elsevier Inc. The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72–96 h and is preceded by a sensitive phase (24–48 h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96 h exposure to 0 or 200 μM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase. Only 11 proteins revealed Al-induced abundance differences; six were identified. After 24 h, phenylalanine ammonium lyase (PAL), methionine synthase (MS), and deoxymugineic acid synthase (DMAS) decreased, while acid phosphatase (APase) abundance increased. Coincident with growth recovering, PAL and MS, but not DMAS, returned to initial levels. After 96 h, γ‑carbonic anhydrase (γ‑CA) and adenylate kinase (AK) along with two unidentified proteins were more abundant. In conclusion, few protein changes characterize the initial response to Al in signalgrass. During the alarm phase, changes are related to P-mobilization, downregulation of Fe-acquisition, reduction of phenolic biosynthesis, and small stimulation of organic acid exudation. After recovering (resistant phase), biosynthesis of phenolics and methionine, but not Fe-mobilization are re-established. Full expression of Al-resistance is characterized by enhanced γ‑CA mediating mitochondrial complex I assembly and increased AK abundance indicating higher root respiration and better provision of ADP and Mg2 +to ATP synthase, respectively. The unidentified proteins and the specific role of γ‑CA in Al resistance of U. decumbens will centre future research.

AB - © 2017 Elsevier Inc. The mechanisms of extreme Al-resistance in Urochloa decumbens are not established. Full resistance expression requires a lag time of 72–96 h and is preceded by a sensitive phase (24–48 h) with Al-induced root growth inhibition. The aim here was to identify key processes of the activation phase of Al-resistance analysing both root exudates and comparative root proteome. Samples were taken after 0, 24 and 96 h exposure to 0 or 200 μM Al. Al-induced stimulation of citrate and oxalate efflux was limited to the sensitive phase. Only 11 proteins revealed Al-induced abundance differences; six were identified. After 24 h, phenylalanine ammonium lyase (PAL), methionine synthase (MS), and deoxymugineic acid synthase (DMAS) decreased, while acid phosphatase (APase) abundance increased. Coincident with growth recovering, PAL and MS, but not DMAS, returned to initial levels. After 96 h, γ‑carbonic anhydrase (γ‑CA) and adenylate kinase (AK) along with two unidentified proteins were more abundant. In conclusion, few protein changes characterize the initial response to Al in signalgrass. During the alarm phase, changes are related to P-mobilization, downregulation of Fe-acquisition, reduction of phenolic biosynthesis, and small stimulation of organic acid exudation. After recovering (resistant phase), biosynthesis of phenolics and methionine, but not Fe-mobilization are re-established. Full expression of Al-resistance is characterized by enhanced γ‑CA mediating mitochondrial complex I assembly and increased AK abundance indicating higher root respiration and better provision of ADP and Mg2 +to ATP synthase, respectively. The unidentified proteins and the specific role of γ‑CA in Al resistance of U. decumbens will centre future research.

U2 - 10.1016/j.jinorgbio.2017.09.010

DO - 10.1016/j.jinorgbio.2017.09.010

M3 - Article

SP - 145

EP - 151

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

SN - 0162-0134

ER -