We have investigated the magnetic, structural and morphological properties of La0.6Sr0.4MnO3 (LSMO-40) manganite particles, synthesized by solid state reaction method. The resulting LSMO-40 powders were milled in air atmosphere during 3, 6 and 12 hours, by using a planetary ball milling. Samples obtained were characterized by X-ray diffraction, scanning electron microscopy-SEM and magnetization measurements as a function of temperature and magnetic field. A Rietveld analysis was carried on each XRD pattern, and was observed a reduction in crystallite average size (Dv) with increased ball milling time, tM. This is associated with a decrease in particle size. A characteristic rhombohedral crystal structure for the LSMO-40 phase was identified (space group R3C), independent of the milling time of the powders. However, from SEM microstructure was observed more homogeneity in the grain distribution by milling process. The results of magnetic characterization, showed that samples with higher tM (smaller grain size), presented the lowest value of the saturation magnetization, which is attributed to surface effects that induce magnetically disordered states with decreasing particle sizes. This magnetic anisotropy surface is evidenced also on the changes of coercive fields, HC, measured at low temperatures, which increased with increasing tM.